The Montreal Biodome is a facility located at Olympic Park in the Montreal neighborhood of Mercier-Hochelaga-Maisonneuve that allows visitors to walk through replicas of four ecosystems found in the Americas. The building was originally constructed for the 1976 Olympic Games as a velodrome (an arena for track cycling). It hosted both track cycling and judo events. Renovations on the building began in 1989 and in 1992 the indoor nature exhibit was opened.

The facility allows visitors to walk through replicas of four ecosystems found in the Americas:

The Tropical Forest is a replica of the South American rain forest.
The Laurentian Forest is a replica of the North American wilderness.
The Saint Lawrence Marine Eco-system is an estuary habitat modeled on the Gulf of Saint Lawrence.
The Sub-Polar Region is a habitat that is divided into Arctic and Antarctic.

All the exhibits are housed inside the former velodrome that was used for the cycling and judo events of the 1976 Summer Olympics, with each of the four environments taking up a portion of the stadium. A variety of animals live in each simulated habitat, ranging from the macaws in the Tropical Forest, to the lynx in the Laurentian Forest, to the penguins in the Antarctic and the different kinds of fish that inhabit the waters of the Saint Lawrence River. As well, two new species have been discovered living in the Biodome: the acarian Copidognathus biodomus in the simulated estuary in 1996, and the bacterium Nitratireductor aquibiodomus in the water reprocessing system in 2003.